University of Delaware - College of Engineering
CHEMICAL & BIOMOLECULAR ENGINEERING

People

Faculty

Anne S Robinson

Anne Robinson
  • Bioengineering Graduate Program
  • Associate Chair for Biochemical Engineering
  • Full Professor


University of Delaware
Newark, Delaware 19716
asr@udel.edu
302-831-0557
302-831-1048 (fax)


Education
  • Doctorate - 1994 University of Illinois at Urbana-Champaign
  • Masters - 1989 The Johns Hopkins University
  • Bachelors - 1988 The Johns Hopkins University

"The Robinson laboratory is interested in understanding the fundamental interactions between molecules, both in isolation and in the complex environment of the cell. We use our growing understanding to design proteins with more robust or novel properties and to engineer cellular systems for improved production or drug screening applications. To this end, we are investigating the determinants of protein folding and misfolding, on both an atomic and molecular level. We have developed several novel approaches to inhibiting protein misfolding and aggregation. Additionally, we are designing cellular systems for optimal expression of membrane proteins, and antibodies.Our approach uses techniques in molecular biology, genetic engineering, and biophysical chemistry to identify and study macromolecules at both an atomic and cellular level. Mechanistic modeling guides us in experimental design and analysis. We use both prokaryotic, or bacterial, systems as well as eukaryotic systems, such as yeast or mammalian cells. A major goal of this research is to establish a set of cellular systems that could express any protein of interest. The research in the laboratory has focus areas in protein stability, expression, and aggregation for biotechnology and biomedical applications. Click HERE to visit our Research Site. "

XSelected Publications

For a complete listing of publications, please view the Full CV.
  1. Spatara, ML and Robinson, AS, "Transgenic mouse and cell culture models demonstrate a lack of mechanistic connection between endoplasmic reticulum stress and tau dysfunction", Journal of Neuroscience Research 88(9), 1951, (2010)
  2. O'Malley MA, Mancini JD, Young CL, McCusker EC, Raden D, Robinson AS, "Progress toward heterologous expression of active G-protein-coupled receptors in Saccharomyces cerevisiae: Linking cellular stress response with translocation and trafficking", Protein Sci. 18(11), 2356, (2009)
  3. Robinson, A.S., "Production of Membrane Proteins ? Strategies for Expression and Isolation", 0
  4. Webber T, Gurung S, Saul J, Baker T, Spatara M, Freyer M, Robinson AS, Gage MJ, "The C-terminus of the P22 tailspike protein acts as an independent oligomerization domain for monomeric proteins", Biochem J (2009)
  5. Xu, P. and Robinson, A.S., "Decreased secretion and unfolded protein response up-regulation are correlated with intracellular retention for single-chain antibody variants produced in yeast", Biotech & Bioeng (2009)
  6. Spatara ML, Roberts CJ, Robinson AS, "Kinetic folding studies of the P22 tailspike beta-helix domain reveal multiple unfolded states", Biophys Chem (2009)
  7. McCusker, E., and Robinson, A.S., "Refolding of G protein ? subunits from inclusion bodies expressed.", Escherichia coli, Protein Exp. Purif. 58(2), 342, (2008)
  8. Russell, T.W.F., Robinson, A.S., and Wagner, N.J., "Mass and Heat Transfer: Analysis of Mass Contactors and Heat Exchangers", (2008)
  9. Hildebrandt, S., D. Raden, L. Petzold, A.S. Robinson, and F.J. Doyle III, "A top-down approach to mechanistic biological modeling: application to the single-chain antibody folding pathway", Biophysical Journal 95(8), 3535, (2008)
  10. Powers, S.L., Robinson, C.R., and Robinson, A.S., "Denaturation of an Extremely Stable Hyperthermophilic Protein Occurs via a Dimeric Intermediate", Extremophiles 11 (1), 179, (2007)

XPatents

  1. Use of Hydrostatic Pressure to Inhibit and Reverse Protein Aggregation and Facilitate Protein Refolding. (Pending 10/99) (60/161,035): Robinson, A.S., D.Foguel, J.L. Silva, C., 1999
  2. Methods for Increasing Secretion of Overexpressed Proteins. (5,773,245): Robinson, A.S. and K.D. Wittrup, 1998
  3. Proteolytic Enzymes from Hyperthermophilic Bacteria and Processes for Their Production. (5,242,817): Kelly, R.M., A.K.S. Robinson, I.I. Blumentals, S.H. Brown, and C.B. Anfinsen, 1993

XSelected Awards

For a complete listing of awards, please view the Full CV.
  1. DuPont Young Professor: 2000 - 2003
  2. NSF Presidential Early Career Award in Science and Engineering: 2000
  3. NIH Postdoctoral Fellowship: 1996 - 1997
  4. Clare Booth Luce Graduate Fellowship: 1992 - 1994
  5. Department of Defense Fellowship ((NDSEG): 1989 - 1992)
  6. NSF Creativity Award for Scientists and Engineers: 1988 - 1989
  7. Tau Beta Pi Fellowship: 1988 - 1989
  8. Beneficial Hodson Scholarship (Johns Hopkins University: 1984 - 1988)
  10. National Academy of Engineering, Sixth Annual Frontiers in Engineering (National Academy of Engineering)


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