Research Program

Current Projects

  • Microbiome
  • E. coli kinetic model
  • Cancer and diabetes
  • CHO cell culture
  • Algae, cyanobacteria

Research Methods

Research Interests

Antoniewicz Laboratory

The Antoniewicz lab is developing next generation tools and techniques for analyzing, engineering and manipulating microbial and mammalian cells applied to specific problems in biotechnology (biofuels, pharmaceuticals) and medicine (cancer, obesity, diabetes). In our research we make use of modern techniques for cell culture, metabolic flux analysis, tandem mass spectrometry, molecular biology, bioinformatics and computational biology.


Current Projects

  • Microbial communities and microbiome
    We are elucidating interactions in synthetic and natural microbial communities using novel 13C-metabolic flux analysis techniques that we are developing in our lab.
    Ph.D. Students: Niko Gebreselassie and Camil Diaz

  • E. coli metabolism, adaptive evolution
    We are studying E. coli metabolism by quantifying cellular responses to genetic perturbations and measuring flux rewiring after adaptive laboratory evolution.
    Ph.D. Student: Chris Long

  • Cancer and diabetes
    We are elucidating the metabolism of cancer cells, liver cells, and fat cells, and identifying novel therapeutic targets for treating cancer and diabetes.
    Ph.D. Student: Eleanor Oates

  • CHO cell culture
    We are studying dynamic metabolism of Chinese hamster ovary (CHO) cells in fed-batch culture and new developing strateggies to improve protein production.
    Ph.D. Student: Jennifer Au

  • Algae and cyanobacteria
    We are studying metabolism of microalgae and cyanobacteria and identifying new metabolic engineering targets to improve CO2 fixation and photosynthetic efficiency.
    Ph.D. Student: Brian McConnell


Research Methods

To accomplish our research goals we make use of a diverse array of scientific tools:

  • Metabolic Engineering
    Experimental techniques for modulating metabolic fluxes, either directly using gene overexpression/knockout techniques, and indirectly by modulating regulation of cellular function using combinatorial methods. [Read More]

  • Systems Biology
    Computational tools for constructing and analyzing genome-scale cellular models, and techniques for analyzing large volumes of biological data generated by microarrays and other "omics" technologies. [Read More]

  • Metabolic Flux Analysis
    Experimental and computational techniques for quantifying metabolic fluxes in vivo using stable-isotope labeling experiments and (tandem) mass spectrometry measurements. These tools are applied to study metabolic reaction networks and control structures in model microbes and mammalian cells. [Read More]

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RESEARCH AREAS

Metabolic Engineering

Metabolic Engineering of Microbes

Tandem Mass Spectrometry

Tandem Mass Spectrometry

Mammalian Cell Culture

Mammalian Cell Culture

Metabolic Flux Analysis

Metabolic Flux Analysis