Cellular & Molecular Engineering Laboratory

Publications

Gage, M.J, Lefebvre, B.G., and A.S. Robinson (2006) “Determinants of Protein Folding and Aggregation in P22 Tailspike,” in Misbehaving Proteins, ACS Publications, eds. Regina Murphy and Amos Tsai. In press.

Niebauer, R. T., and A.S. Robinson (2005) “Exceptional total and functional yields of the human adenosine (A2a) receptor expressed in the yeast Saccharomyces cerevisiae”, Prot. Exp. Purif., in press.

Raden, D., Hildebrandt, S., Xu, P., Bell, E., Doyle III, F. J., and Robinson, A. S. (2005) "Analysis of the cellular response to protein overexpression." Systems Biology (Stevenage) 152(4), 285-289.

Xu, P., Raden, D., Doyle, F., and Robinson, A. (2005) "Analysis of unfolded protein response during single-chain antibody expression in saccaromyces cerevisiae reveals different roles for bip and pdi in folding." Metab Eng 7, 4, 269–79.

Smith, J., Richardson, N., and Robinson, A. (2005) "Elevated expression temperature in a mesophilic host results in increased secretion of a hyperthermophilic enzyme and decreased cell stress." Biochim Biophys Acta 1752, 1, 18–25.

Gage, M., Zak, J., and Robinson, A. (2005) "Three amino acids that are critical to formation and stability of the p22 tailspike trimer." Protein Sci 14, 9, 2333–43.

Danek, B., and Robinson, A. (2004) "P22 tailspike trimer assembly is governed by interchain redox associations." Biochim Biophys Acta 1700, 1, 105–16.

Lefebvre, B., Comolli, N., Gage, M., and Robinson, A. (2004) "Pressure dissociation studies provide insight into oligomerization competence of temperature-sensitive folding mutants of p22 tailspike." Protein Sci 13, 6, 1538–46.

Niebauer, R., Wedekind, A., and Robinson, A. (2004) "Decreases in yeast expression yields of the human adenosine a2a receptor are a result of translational or post-translational events." Protein Expr Purif 37, 1, 134–43.

Smith, J., Tang, B., and Robinson, A. (2004) "Protein disulfide isomerase, but not binding protein, overexpression enhances secretion of a non-disulfide-bonded protein in yeast." Biotechnol Bioeng 85, 3, 340–50.

Danek, B., and Robinson, A. "Nonnative interactions between cysteines direct productive assembly of p22 tailspike protein." Biophys J 85, 5 (2003), 3237–47.

Gage, M., and Robinson, A. "C-terminal hydrophobic interactions play a critical role in oligomeric assembly of the p22 tailspike trimer." Protein Sci 12, 12 (2003), 2732–47.

Butz, J. and A.S. Robinson (2003), “Interaction with ER-resident Proteins is not a Bottleneck for Mammalian G-Protein Coupled Receptor Expression in Yeast,” Biotech. Bioeng., 84, 3, p. 292-304. DOI: 10.1002/bit.10771.

Lefebvre, B.G., and A.S. Robinson (2003), “Pressure treatment of tailspike aggregates rapidly produces on-pathway folding intermediates,” Biotech. Bioeng, 82, 5, p. 595-604.

Sinacola, J. and A.S. Robinson (2002) “Rapid refolding and polishing of single-chain antibodies from E. coli inclusion bodies” Protein Exp. Purif., Vol. 26, No. 2, Nov 2002, pp. 301-308.

Smith, J.D. and A.S. Robinson (2002) “Expression of an archael enzyme in aeucaryotic host: A secretion bottleneck at the ER,” Biotech. Bioeng., 79, 7, p. 713-723.

Kauffman, K., Pridgen, E.M., Doyle, F.J. III, Dhurjati, P., and A.S. Robinson (2002) “Decreased Protein Expression and Oscillating BiP Levels Result during Heterologous Protein Expression in S. cerevisiae,” Biotech. Prog., 18, 942-940.

Haase-Pettingell, C., Betts, S., Raso, S.W., Stuart, L., Robinson, A.S. and J. King (2001), Role for Cysteine Residues in the In Vivo Folding and Assembly of the Page P22 Tailspike, Protein Science, 2001, 10:397-410. PDF version

Kauffman, K., P. Dhurjati, A.S. Robinson, and F.J. Doyle III, “Framework for Modeling Information Flow in Biological Processes: Application to the Unfolded Protein Response.” Proc. IFAC Conf. Comput. Appl. Biotech (CAB), 2001.

Foguel, D., Robinson, C.R., Caetano de Sousa Jr., P., Silva, J. L. and A. S. Robinson (1999), Hydrostatic Pressure Rescues Protein Aggregates, Biotechnology & Bioengineering. 63, Pp. 552-558. PDF version

Robinson, A.S. and J. King, (1997) Disulfide-Bonded Intermediate on the Folding and Assembly Pathway of a Non-Disulfide Bonded Protein. Nature Structural Biology, 4, Pp. 450-455. Abstract

King, J., C. Haase-Pettingell, A.S. Robinson, M. Speed, and A. Mitraki (1996) Thermolabile Folding Intermediates: Inclusion Body Precursors and Chaperonin Substrates. FASEB Journal, 10, Pp. 57-66.

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