the wagner group

Daniel Greene

Curriculum Vitae
B.S. Chemical Engineering, 2011
University of Massachusetts, Amherst, MA

office: 155 Colburn Lab
phone: 302-831-6642

Dense Phase Protein Behavior
Co-advised by Dr. Abraham Lenhoff

The phase behavior of native protein solutions is important in many industrial processes such as crystallization and precipitation and can affect processing via the presence of aggregates. The behavior is a direct consequence of the intermolecular interactions which can be quite complex and depend on many variables (i.e. concentration, temperature, pH, etc.), manifesting itself in a high dimensional phase diagram. Because of the complexity, very little work has been done to characterize these dense phases. Understanding the structure of these phases will yield information about the mechanism by which they form and thus potentially lead to a ˇ°smarterˇ± design of various processes. Using scattering techniques and Cyro-SEM, the structure of dense protein phases will be examined.