PUBLICATIONS

225. Daniel Blackstock and Wilfred Chen, Halo-tag mediated self-labeling of fluorescent proteins to molecular beacons for nucleic acid detection, Chem. Comm., Accepted for Publication.

224. Daniel Blackstock, Qing Sun, and Wilfred Chen, Fluorescent protein-based molecular beacons by zinc finger protein-guided assembly, Biotechnol. Bioeng., Accepted for Publication.

223. Miso Park, Qing Sun, Fang Liu, Matthew P. DeLisa and Wilfred Chen, Positional assembly of enzymes on bacterial outer membrane vesicles for cascade reactions, PLOS one, 9, e97103, 2014. pdf

222. Kishore K. Krishnani, Wilfred Chen, and Ashok Mulchandani, Bactericidal activity of elastin-like polypeptide biopolymer with polyhistidine domain and silver, Colloids and Surfaces B: Biointerfaces, 119, 66-70, 2014. pdf

221. Rahul D. Sheth, Mi Jin, Bharat V. Bhut, Jia Liu, Jongchan Lee, Rieble Siegfried, Zhengjian Li, Wilfred Chen, Steven M. Cramer, Affinity precipitation of monoclonal antibody from industrial harvest feedstock using ELP-Z stimuli responsive biopolymers, Biotechnol. Bioeng., 111, 1595-1603, 2014. pdf

220. Lakshmi N Cella, Payal Biswas, Marylynn V. Yates, Ashok Mulchandani, Wilfred Chen, Quantitative assessment of in vivo HIV protease activity using genetically engineered QD-based FRET probes, Biotechnol. Bioeng. 111, 1082-1087, 2014. pdf

219. Qing Sun, Bhawna Madan, Shen-Long Tsai, Matthew P. DeLisa and Wilfred Chen, Creation of artificial cellulosomes on DNA scaffolds by zinc finger protein-guided assembly for efficient cellulose hydrolysis, Chem. Comm., 50, 1423-1425, 2014. pdf

218. Rebecca Chen, Qi Chen, Heejae Kim, Ka-Hei Siu, Qing Sun, Shen-Long Tsai and Wilfred Chen, Biomolecular Scaffolds for Enhanced Signaling and Catalytic Efficiency, Curr. Opin. Biotechnol., 28, 59-68, 2014. pdf

217. Daniel Blackstock, Miso Park, Qing Sun, Shen-Long Tsai, and Wilfred Chen, Engineering protein modules for diagnostic applications, Curr. Opin. Chem. Eng., 2, 416-424, 2013. pdf

216. Fang Liu and Wilfred Chen, Engineering a recyclable ELP capturing scaffold for non-chromatographic protein purification, Biotechnol Prog. 29, 968-971, 2013. pdf

215. Miso Park, Shen-Long Tsai, and Wilfred Chen, Microbial biosensors: Engineered microorganisms as the sensing machinery, Sensors, 13, 5777-5795, 2013. pdf

214. Nicha Chartuprayoon, Youngwoo Rheem, James C. K. Ng, Jin Nam, Wilfred Chen, and Nosang V. Myung, Polypyrrole nanoribbon based chemiresistive immunosensor for viral plant pathogen detection, Anal. Methods, 5, 3497-3502, 2013. pdf

213. Rahul D. Sheth, Bhawna Madan, Wilfred Chen, Steven M. Cramer, High throughput screening for the development of a monoclonal antibody affinity precipitation step using ELP-Z stimuli responsive biopolymers, Biotechnol. Bioeng., 110, 2664-2676, 2013. pdf

212. Fang Liu, Scott Banta, and Wilfred Chen, Functional assembly of a multi-enzyme methanol oxidation cascade on a surface-displayed trifunctional scaffold for enhanced NADH production, Chem. Comm., 49, 3766-3768, 2013. pdf

211. Lakshmi N Cella, Daniel Blackstock, Marylynn A. Yates, Ashok Mulchandani, Wilfred Chen, Detection of RNA Viruses: Current technologies and Future perspectives, Critical Reviews in Eukaryotic Gene Expression, 23, 125-137, 2013. pdf

210. Divya Sivaraman, Hsiao-Yun Yeh, Ashok Mulchandani, Marylynn V. Yates and Wilfred Chen, Use of flow cytometry for the rapid and quantitative detection of poliovirus-infected cells via TAT peptide-delivered molecular beacons, Appl. Environ. Microbiol., 79, 696-700, 2013 . pdf  Highlight

209. Bhawna Madan, Garima Chaudhary, Steven M. Cramer, and Wilfred Chen, ELP-z and ELP-zz capturing scaffolds for the purification of immunoglobulins by affinity precipitation, J. Biotechnol. 163, 10-16, 2013. pdf

208. Heejae Kim, Qing Sun, Fang Liu, Shen-Long Tsai, and Wilfred Chen, Biologically assembled nanobiocatalysts, Topics in Catalysis, 55, 1138-1145, 2012. pdf

207. Shen-Long Tsai, Miso Park, Wilfred Chen, Size-modulated synergy of cellulase clustering for enhanced cellulose hydrolysis, Biotechnol. J., 8, 257–261, 2013. pdf

206. Shen-Long Tsai, Nancy A. DaSilva, and Wilfred Chen, Functional display of complex cellulosomes on the yeast surface via adaptive assembly, ACS Synthetic Biology, 2, 14-21, 2013. pdf

205. Sneha Srikrishnan, Wilfred Chen, and Nancy A. Da Silva, Functional assembly and characterization of a modular xylanosome for hemicellulose hydrolysis in yeast, Biotechnol. Bioeng., 110, 275–285, 2013. pdf

204. Fang Liu, Shen-Long Tsai, Bhawna Madan, and Wilfred Chen, Engineering a high-affinity scaffold for non-chromatographic protein purification via intein-mediated cleavage, Biotechnol. Bioeng., 109, 2829–2835, 2012. pdf

203. Maxwell C. Kum, Hyunsung Jung, Nicha Chartuprayoon, Wilfred Chen, Ashok Mulchandani, and Nosang V. Myung, Tuning electrical and optoelectronic properties of single cadmium telluride nanoribbon, J. Phys. Chem., 116, 9202-9208, 2012. pdf

202. Michael W.Y. Shen, Dhawal Shah, Wilfred Chen, Nancy Da Silva, Enhanced arsenate uptake in yeast engineered for overexpression of the pho84 phosphate transporter, Biotechnol. Prog., 28, 654-661, 2012. pdf

201. Rajwant S. Bedi, Rui Cai, Cory O’Neill, Derek E. Beving, Stephen Foster, Sean Guthrie, Wilfred Chen, and Yushan Yan, Hydrophilic and antimicrobial Ag-exchanged zeolite A coatings: A year-long durability study and preliminary evidence for their general microbiocidal efficacy to bacteria, fungus and yeast, Microporous and Mesoporous Materials, 151, 352-357, 2012. pdf

200. Garima Goyal, Shen Long Tsai, Bhawna Madan, Nancy Da Silva, and Wilfred Chen, Simultaneous cell growth and ethanol production from cellulose by an engineered yeast consortium displaying a functional mini-cellulosome, Microb. Cell Factories, 10, 89, 2011. pdf

199. Daniela Dunams, Payal Sarkar, Wilfred Chen, and Marylynn Yates, Simultaneous detection of infectious human echoviruses and adenoviruses by an in situ nuclease-resistant molecular beacon-based assay, Appl. Environ. Microbiol., 78, 1548-1588, 2012. pdf

198. Xiaojian Chen, Wilfred Chen, Ashok Mulchandani, and Umar Mohideen, Application of displacement principle for detecting heavy metal ions and EDTA using microcantilever, Sensors & Actuators: B. Chemical, 161, 203-208, 2012. pdf

197. Shen-Long Tsai, Shailendra Singh, Nancy A. DaSilva, and Wilfred Chen, Co-expression of Arabidopsis thaliana phytochelatin synthase and Treponema denticola cysteine desulfhydrase for enhanced arsenic accumulation, Biotechnol. Bioeng., 109, 605–608, 2012. pdf

196. Joun S. Lee, Syed Mubeen, Carlos M. Hangarter, Ashok Mulchandani, Wilfred Chen, and Nosang V. Myung, Selective and rapid room temperature detection of H2S using gold nanoparticle chain arrays, Electroanalysis, 23, 2623-2628, 2011. pdf

195. Shenghua Jiang, Fang Liu, Min-Gyu Kim, Jae-Hong Lim, Kun-Jae Lee, Yong-Ho Choa, Kyung Song, Michael J. Sadowsky, Wilfred Chen, Nosang V. Myung, and Hor-Gil Hur, Synthesis of chalcogenide ternary and quaternary nanotubes through directed compositional alterations of bacterial As-S nanotubes, J. of Materials Chemistry, 21, 10277-10279. 2011. pdf 

194. Payal Ganguli, Wilfred Chen, and Marylynn V. Yates, Detection of Murine Norovirus-1 using TAT peptide delivered molecular beacon, Appl. Environ. Microbiol., 77, 5517-5520, 2011. pdf

193. Payal Biswas, Lakshmi N Cella, Seung Hyun Kang, Ashok Mulchandani, Marylynn V. Yates, and Wilfred Chen, A quantum-dot based protein module for in vivo monitoring of protease activity through fluorescence resonance energy transfer, Chem. Comm., 47, 5259–5261, 2011. pdf

192. Divya Sivaraman, Payal Biswas, Lakshmi N Cella, Marylynn V. Yates and Wilfred Chen, Detecting RNA viruses in living mammalian cells by fluorescence microscopy, Trends in Biotechnol., 29, 307-313, 2011. pdf

191. Basanta K. Das, Chaker Tlili, Sushmee Badhulika, Lakshmi N. Cella, Wilfred Chen, and Ashok Mulchandani, Single-walled carbon nanotubes chemiresistor aptasensors for small molecules: picomolar level detection of adenosine triphosphate, Chem. Comm., 47, 3793–3795, 2011. pdf

190. Jason L. Cantera, Wilfred Chen, and Marylynn V. Yates, A fluorescence resonance energy transfer-based fluorometer assay for screening for anti-coxsackievirus B3 compounds, J. Virol. Methods, 171, 176-182, 2011. pdf

189. Dhammanand J. Shirale, Mangesh A. Bangar, Miso Park, Marylynn V. Yates, Wilfred Chen, Nosang V. Myung and Ashok Mulchandani, Label-Free Chemiresistive Immunosensors for Viruses, Environ. Sci. Technol., 44, 9030-9035, 2010. pdf

188. Mangesh A. Bangar, Dhammanand J. Shirale,  Hemant Purohit, Wilfred Chen,  Nosang V. Myung, and Ashok Mulchandani, Single Conducting Polymer Nanowire Based Sequence-Specific, Base-Pair-Length Dependent Label-free DNA Sensor, Electroanal., 23, 371-379, 2011. pdf

187. Manngesh A. Banger, Wilfred Chen, Nosang V. Myung, and Ashok Mulchandani, Conducting polymer 1-dimensional nanostructures for FET sensors, Thin Solid Films, 519, 964-973, 2010. pdf

186.  Shen-Long Tsai, Garima Goyal, and Wilfred Chen, Surface display of a functional mini-cellulosome by intracellular complementation using a synthetic yeast consortium and its application to cellulose hydrolysis and ethanol production, Appl. Environ. Microbiol., 76, 7514-7520, 2010. pdf

185. Fang Liu, Seung Hyun Kang, Young-In Lee, Yong-ho Choa, Ashok Mulchandani, Nosang V. Myung, and Wilfred Chen, Enzyme mediated synthesis of phytochelatin-capped CdS nanocrystals, Appl. Phys. Letts., 97, 123703, 2010. pdf

184. Miso Park, Lakshmi N Cella, Wilfred Chen, Nosang V. Myung, and Ashok Mulchandani, Carbon nanotubes-based chemiresistive immunosensor for small molecules: Detection of nitroaromatic explosives, Biosensors and Bioelectronics, , 26, 1297-1301, 2010. pdf

183. Dhammanand J. Shirale, Mangesh A. Bangar, Wilfred Chen, Nosang V. Myung and Ashok Mulchandani, Effect of Aspect Ratio (Length : Diameter) on a Single Polypyrrole Nanowire FET Device, J. Phys. Chem. C, 114, 13375-13380, 2010. pdf

182. Dhawal Shah, Michael W.Y. Shen, Wilfred Chen, Nancy A. Da Silva, Enhanced arsenic accumulation in Saccharomyces cerevisiae overexpressing transporters Fps1p or Hxt7p, J. Biotechnol., 150, 101-107, 2010. pdf

181. Hsiao-Yun Yeh, Marylynn V. Yates, Ashok Mulchandani, and Wilfred Chen, Molecular beacon-quantum dot-Au nanoparticle hybrid nanoprobes for visualizing virus replication in living cells, Chem. Comm., 46, 3914–3916, 2010. pdf

180. Lakshmi Cella, Wilfred Chen, Nosang V. Myung, and Ashok Mulchandani, Single-Walled Carbon Nanotubes-Based Chemiresistive Affinity Biosensors for Small Molecules: Ultrasensitive Glucose Detection, J. Am. Chem. Soc., 132, 6024-5026, 2010. pdf

179. Lakshmi Cella, Pablo Sanchez, Wenwan Zhong, Nosang V. Myung, Wilfred Chen, Ashok Mulchandani, Nano aptasensor for protective antigen toxin of Anthrax, Anal. Chem., 82, 2042-2047, 2010. pdf

178. Kawai Tam, Cuong Tu, Jin-Hoon Lee, Min Lai, Chong Hyun Chang, Youngwoo Rheem, Wilfred Chen, Hor-Gil Hur, and Nosang V. Myung, Growth Mechanism of Amorphous Selenium Nanoparticles Synthesis by Shewanella sp. HN-41, Bioscience, Biotechnology and Biochemistry, 74, 696-700, 2010. pdf

177. Shailendra Singh, Krassimir Bozhilov, Ashok Mulchandani, Nosang Myung, and Wilfred Chen, Biologically Programmed Synthesis of Core-Shell CdSe/ZnS Nanocrystals, Chem. Comm., 46, 1473-1475, 2010. pdf

176. Jason L. Cantera, Wilfred Chen, and Marylynn V. Yates, Detection of infective Poliovirus by a simple, rapid, and sensitive flow cytometry method based on fluorescence resonance energy transfer technology, Appl. Environ. Microbiol., 76, 584-588, 2010. pdf

175. Shailendra Singh, Seung Hyun Kang, Wonkyu Lee, Ashok Mulchandani, and Wilfred Chen, Systematic Engineering of Phytochelatin Synthesis and Arsenic Transport for Enhanced Arsenic Accumulation in E. coli, Biotechnol. Bioeng., 105, 780–785, 2010. pdf

174. Shen-Long Tsai, Shailendra Singh, and Wilfred Chen, Arsenic Metabolism by Microbes in Nature and the Impact on Arsenic Remediation, Curr. Opin. Biotechnol., 20, 659–667, 2009. pdf

173. Shen-Long Tsai, Jeongseok Oh, Shailendra Singh, Ruizhen Chen, and Wilfred Chen, Functional assembly of mini-cellulosomes on the yeast surface for cellulose hydrolysis and ethanol production, Appl. Environ. Microbiol., 75, 6087-6093, 2009. pdf

172. Zheng Liu, Chao Yang, Hong Jiang, Ashok Mulchandani, Wilfred Chen, and Chuanling Qiao, Simultaneous Degradation of Organophosphates and 4-Substituted Phenols by Stenotrophomonas Species LZ-1 with Surface-Displayed Organophosphorus Hydrolase, J. Agric. Food Chem., 51, 6171-6177, 2009. pdf

171. Cindy H. Wu, Stéphanie M. Bernard, Gary L. Andersen and Wilfred Chen, Developing microbe–plant interactions for applications in plant-growth promotion and disease control, production of useful compounds, remediation and carbon sequestration, Microbial Biotechnology, 2, 428–440, 2009. pdf

170. Mangesh A. Bangar, D. J. Shirale, Wilfred Chen, Nosang V. Myung and Ashok Mulchandani, Single conducting polymer nanowire chemiresistive label-free immunosensor for cancer biomarker, Anal. Chem., 81, 2168–2175, 2009. pdf

169. Huimin Zhao and Wilfred Chen, Chemical biotechnology: microbial solutions to global change, Curr. Opin. Biotechnol., 19, 541–543, 2008. pdf

168. Hsiao-Yun Yeh, Marylynn V. Yates, Wilfred Chen, and Ashok Mulchandani, Real-time molecular methods to detect infectious viruses: a mini-review, Seminars in Cell & Developmental Biology, 20, 49–54, 2009. (Invited Contribution) pdf

167. Cindy H. Wu, David Le, Ashok Mulchandani, and Wilfred Chen, Optimization of a whole-cell cadmium sensor with a toggle gene circuit, Biotechnol. Prog., 25, 898–903, 2009. pdf

166. Chao Yang, Yaran Zhu, Jijan Yang, Zheng Liu, Chuanling Qiao, Ashok Mulchandani, and Wilfred Chen, Development of an autofluorescent whole-cell biocatalyst by displaying dual functional moieties on Escherichia coli and construction of a co-culture with organophosphate mineralizing activity, Appl. Environ. Microbiol., 74, 7733–7739, 2008. pdf

165. Mangesh A. Banger, Carlos M. Hangarter, Bongyoung Yoo, Youngwoo Rheem, Wilfred Chen, Ashok Mulchandani, and Nosang Myung, Magnetically assembled multi-segmented nanowires and their applications, Electroanalysis, 21, 61 – 67, 2009. pdf

164. Hsiao-Yun Yeh, Marylynn V. Yates, Ashok Mulchandani, and Wilfred Chen, Visualizing the dynamics of viral replication in living cells via TAT-peptide delivery of nuclease-resistant molecular beacons, Proc. Natl. Acad. Sci. USA, 105, 17522-17525, 2008. pdf

163. C.L. Aravinda, Serge Cosnier, Wilfred Chen, Nosang V. Myung, and Ashok Mulchandani, Label-free detection of cupric ions and histidine-tagged proteins using single poly(pyrrole)-NTA chelator conducting polymer nanotube chemi-resistive sensor, Biosensors and Bioelectronics, 24, 1451–1455, 2009. pdf

162. Shailendra Singh, Seung Hyun Kang, Ashok Mulchandani and Wilfred Chen, Bioremediation: Environmental clean up through pathway engineering, Curr. Opin. Biotechnol., 19, 437–444, 2008. (Invited Contribution) pdf

161. Chao Yang, Qiao Zhao, Zheng Liu, Qiyun Li, Chuanling Qiao, Ashok Mulchandani, and Wilfred Chen, Cell surface display of functional macromolecule fusions on Escherichia coli for development of a bioluminescent whole-cell biocatalyst, Environ. Sci. Technol., 42, 6105-6110, 2008. pdf

160. Maxwell C. Kum, Bong Young Yoo, Young Woo Rheem, Krassimir N. Bozhilov, Wilfred Chen, Ashok Mulchandani, and Nosang V. Myung, Synthesis and characterization of cadmium telluride nanowire, Nanotechnology, 19, 325711, 2008. pdf

159. Seung Hyun Kang, Krassimir N. Bozhilov, Nosang V. Myung, Ashok Mulchandani and Wilfred Chen, Microbial synthesis of CdS nanocrystals in genetically engineered E. coli, Angewandte Chemie International Edition, 47, 5186 –5189, 2008. pdf

158. Adam K. Wanekaya, Wilfred Chen, Ashok Mulchandani, Recent biosensing developments in environmental security, J. Environ. Monitoring, 10, 703–712, 2008. pdf

157. S. Sandhya, Wilfred Chen and Ashok Mulchandani, Molecular Beacons: A Real-Time Polymerase Chain Reaction Assay for Detecting Escherichia coli from Fresh Produce and Water, Analytica Chimica Acta, 614, 208-212, 2008. pdf

156. Lianbin Xu, Zhongwei Chen, Wilfred Chen, Ashok Mulchandani, and Yushan Yan, Electrochmical synthesis of perfluorinated ions doped coducting polyaniline films consisting of helical fibers and their reversible switching between superhydrophobicity and superhydrophilicity, Macromolecular Rapid Communications, 29, 832–838, 2008. pdf

155. Cindy H. Wu, Yu-Chen Hwang, Wonkyu Lee, Ashok Mulchandani, Thomas K. Wood, Marylynn V. Yates, and Wilfred Chen, Detection of recombinant Pseudomonas putida biofilm in wheat rhizosphere by two-colored fluorescence in situ hybridization, Appl. Microbiol. Biotechnol., 79, 511–518, 2008. pdf

154. Shailendra Singh, Ashok Mulchandani, and Wilfred Chen, Highly Selective and Rapid Arsenic Removal by Metabolically Engineered E. coli Expressing Fucus vesiculosus Metallothionein, Appl. Environ. Microbiol., 74, 2924–2927, 2008. pdf

153. Hsiao-Yun Yeh, Yu-Chen Hwang, Marylynn V. Yates, Ashok Mulchandani, and Wilfred Chen, Detection of Hepatitis A Virus By Using a Combined Cell-Culture – Molecular Beacon Assay, Appl. Environ. Microbiol., 74, 2239-2243, 2008. pdf

152. Cindy H. Wu, Ashok Mulchandani, and Wilfred Chen, Versatile microbial surface-display for environmental remediation and biofuels production, Trends in Microbiology, 16, 181-188, 2008. pdf  (Invited Contribution)

151. Carlos N. Hangarter, Mangesh Banger, Sandra C. Hernandez, Wilfred Chen, Marc A. Deshusses, Ashok Mulchandani, and Nosang V. Myung, Maskless electrodeposited contact for conducting polymer nanowires, Appl. Phys. Letts, 92, 073104. 2008. pdf

150. Yu-Chen Hwang, Justin Jang-Hann Chu, Priscilla L. Yang, Wilfred Chen, and Marylynn V. Yate, A Cellular Sensor for Screening Small Molecules Suppressing Poliovirus Replication, Antiviral Research, 77, 232–236, 2008. pdf

149. U Loi Lao, Minwei Sun, Mark Matsumoto, Ashok Mulchandani, and Wilfred Chen, Genetic engineering of self-assembled protein hydrogel based on elastin-like sequences with metal binding functionality, Biomacromolecules, 8, 3736-3739, 2007. pdf

148. Maxwell Kum, Kanchan A. Joshi, Wilfred Chen, Nosang V. Myung, and Ashok Mulchandani, Biomolecules-carbon nanotubes doped conducting polymer composites and their sensor application, Talanta, 74, 370-375, 2007. pdf

147. Joun S. Lee, Aijun A. Wang, Bongyoung Yoo, Youngwoo Rheem, Ashok Mulchandani, Wilfred Chen and Nosang V. Myung, DNA Assisted Assembly of Multi-Segmented Nanowires, Electroanalysis, 22, 2287-2293, 2007. pdf

146. Seung Hyun Kang, Shailendra Singh, Jae-Young Kim, Wonkyu Lee, Ashok Mulchandani, and Wilfred Chen, Bacteria Metabolically Engineered for Enhanced Phytochelatin Production and Cadmium Accumulation, Appl. Environ. Microbiol., 73, 6317-6320, 2007. pdf

145. Shailendra Singh, Wonkyu Lee, Nancy A. DaSilva, Ashok Mulchandani, and Wilfred Chen, Enhanced arsenic accumulation and removal by engineered yeast cells expressing Arabidopsis thaliana phytochelatin synthase, Biotechnol. Bioeng., 99, 333-340, 2008. pdf

144. Can Attila, Akihiro Ueda, Suat L.G. Cirillo, Jeffery D. Cirillo, Wilfred Chen, and Thomas K. Wood, Pseudomonas aeruginosa PAO1 virulence factors and poplar tree response in the rhizosphere, Microbial Biotechnology, 1, 17-29, 2008. pdf

143. Sandra C. Hernandez, Debangshu Chaudhuri, Wilfred Chen, Nosang Myung, and Ashok Mulchandani, Single polypyrrole nanowire ammonia gas sensor, Electroanalysis, 19, 2125-2130, 2007. pdf

142. Chaokun Li, Yaran Zhu, Inga Benz, M. Alexander Schmidt, Wilfred Chen, Ashok Mulchandani, and Chuanling Qiao, Presentation of Functional Organophosphorus Hydrolase Fusions on the Surface of Escherichia coli by the AIDA-I Autotransporter Pathway, Biotechnol. Bioeng., 99, 485-490, 2008. pdf

141. Chao Yang, Ning Cai, Ming Dong, Hong Jiang, Jinming Li, Chuanling Qiao, Ashok Mulchandani, and Wilfred Chen, Surface Display of MPH on Pseudomonas putida JS444 Using Ice Nucleation Protein and Its Application in Detoxification of Organophosphate, Biotechnol. Bioeng., 99, 30-37, 2008. pdf

140. U Loi Lao, Alin Chen, Mark R. Matsumoto, Ashok Mulchandani, Wilfred Chen, Cadmium Removal from Contaminated Soil by Thermally Responsive Elastin (ELPEC20) Biopolymer, Biotechnol. Bioeng., 98, 349-355, 2007. pdf

139. U Loi Lao, Jan Kostal, Ashok Mulchandani, and Wilfred Chen, Affinity Purification of Plasmid DNA by Temperature-Triggered Precipitation, Nature Protocols, 2, 1263-1268, 2007. (Invited Contribution) pdf

138. Y-Z Zheng, W-S. Lan, C-L. Aiao, A. Mulchandani, and W. Chen, Decontamination of vegetables sprayed with organophosphate pesticides by organophosphours hydrolase and carboxylesterase (B1), Appl. Biochem. Biotechnol., 136, 233-242, 2007. pdf

137. Yu Lei, Priti Mulchandani, Wilfred Chen, and Ashok Mulchandani, Biosensor for direct determination of Fenitrothion and EPN using recombinant Pseudomonas putida JS444 with surface-expressed organophosphorous hydrolase, Appl. Biochem. Biotechnol., 136, 243-250, 2007. pdf

136. Yu-Chen Hwang, Oymon M. Leong, Wilfred Chen, and Marylynn V. Yates, Comparison of a Reporter Assay and Immunomagnetic Separation Real-Time RT-PCR for the Detection of Enteroviruses in Seeded Environmental Water Sample, Appl. Environ. Microbiol., 73, 2338-2340, 2007. pdf

135. Adam Wanekaya, Mangesh A. Banger, Minhee Yun, Wilfred Chen, Nosang V. Myung, and Ashok Mulchandani, Field-Effect Transitors Based on Single Nanowires of Conducting Polymers, J. Phys. Chem. C, 111, 5218-5221, 2007. pdf

134. Gabriela Jenikova, U Loi Lao, Di Gao, Ashok Muchaldani and Wilfred Chen, Elastin-Calmodulin Scaffold for Protein Microarray Fabrication, Langmuir, 23, 2277-2279, 2007. pdf

133. Di Gao, Wilfred Chen, Ashok Mulchandani, and Jerome S. Schultz, Detection of Tumor Markers Based on Extinction Spectra of Visible Light Passing through Gold Nanoholes, Appl. Phys. Letts, 90, 073901, 2007. pdf

132. Kumaran Ramanathan, Mangesh A. Bangar, Minhee Yun, Wilfred Chen, Ashok Mulchandani, and Nosang V.  Myung, In Situ Fabricaton of Single Poly(methyl pyrrole) Nanowire, Electroanalysis, 19, 793-797, 2007. pdf

131. Xianpeng Cai, Ningyi Liu, Yu Lei, Qing Zhang, Mary B. Chan, Changming Li, Wilfred Chen, and Ashok Mulchandani, Single-walled carbon nanotube based real-time organophosphate detector, Electroanalysis, 19, 616-619, 2007 pdf

130. Chandana Karnati, Hongwei Du, Hai-Feng Ji, Yuri Lvov, Wilfred Chen, Ashok Mulchandani, Organophosphrus Hydrolyase Multilayer Modified Microcantilevers for Organophosphorus Detection, Biosensor & Bioelectronics, 22, 2636-2642, 2007. pdf

129. W.S. Lan, J.D. Gu, J.L. Zhang, B.C. Shen, H. Jiang, A. Mulchandani, W. Chen, C.L. Qiao, Coexpression of two detoxifying pesticide-degrading enzymes in a genetically engineered bacterium, International Biodeterioration & Biodegradation, 58, 70–76, 2006. pdf

128. U Loi Lao, Ashok Mulchandani, and Wilfred Chen, Simple Conjugation and Purification of Quantum Dot-Antibody Complexes Using A Thermally Responsive Elastin-Protein L Scaffold As Immunofluorescent Agents, J. Am. Chem. Soc., 128, 14756-14757, 2006.  pdf

127. Aijun A. Wang, Joun Lee, Gabriela Jenikova, Ashok Mulchandani, Nosang V. Myung, and Wilfred Chen, Controlled Assembly of Multi-Segment Nanowires by Histidine-Tagged Peptides, Nanotechnology, 17, 3375-3379, 2006. pdf

126. Yu Lei, Priti Mulchanani, Wilfred Chen, and Ashok Mulchandani, Biosensor for direct determination of Fenitrothion and EPN using recombinant Pseudomonas putida JS444 with surface expressed organophosphorus hydrolase. 1. Modified clark oxygen electrode, Sensors, 6, 466-472, 2006. pdf

125. Jintae Lee, Li Cao, Saw Yen Ow, Martin E. Barrios-Lierena, Wilfred Chen, Thomas K. Wood, and Philip C. Wright, Proteome Changes after Metabolic Engineering to Enhance Aerobic Mineralization of cis-1,2-Dichloroethylene, J. Proteome Research, 5, 1388-1397, 2006. pdf

124. Katsumi Takayama, Shin-ichiro Suye, Kouichi Kuroda, Mitsuyoshi Ueda, Tetsuya Kitaguchi, Kouta Tsuchiyama, Takeshi Fukuda, Wilfred Chen, and Ashok Mulchandani, Surface Display of Organophosphorus Hydrolase on Saccharomyces cerevisiae, Biotechnol. Prog., 22, 939-943, 2006. pdf

123. Adam Wanekeya, Yu Lei, Elena Bekyarova, Wilfred Chen, Robert Haddon, Ashok Mulchandani, and Nosang V. Myung, Fabrication and properties of conducting polypyrrole/SWNT-PABS composite films and nanotubes, Electroanalysis, 11, 1047-1054, 2006. pdf

122. Wonkyu Lee, Thomas K. Wood, and Wilfred Chen, Engineering TCE-Degrading Rhizobacteria for Heavy Metal Accumulation and Enhanced TCE Degradation, Biotechnol. Bioeng., 95, 399-403, 2006. pdf

 121. Yu-Chen Hwang, Wilfred Chen, and Marylynn V. Yates, Using Fluorescence Resonance Energy Transfer for Rapid Detection of Enteroviral Infection In Vivo, Appl. Environ. Microbiol., 72, 3710-3715, 2006. pdf

120. Li Cao,  Jintae Lee, Wilfred Chen, and Thomas K. Wood, Enantioconvergent Production of (R)-1-Phenylethane-1,2-Diol from Styrene Oxide by Combining the Solanum tuberosum and an Evolved Agrobacterium radiobacter AD1 Epoxide Hydrolase, Biotechnol. Bioeng., 94, 522-529, 2006. pdf

119. Ayman H. Mansee, Wilfred Chen, and Ashok Mulchandani, Detoxification of the organophosphate nerve agent coumaphos using organophosphorus hydrolase immobilized on cellulose materials, J. Ind. Microbiol. Biotechnol., 32, 554-560, 2005. pdf

118. Adam K. Wanekaya, Wilfred Chen, Nosang V. Myung, and Ashok Mulchandani, Nanowire-Based Electrochemical Biosensor, Electroanalysis, 18, 533-550, 2006. pdf

117. Di Gao, Nicole McBean, Jerome S. Schultz, Yushan Yan,  Ashok Mulchandani, Wilfred Chen, Fabrication of Antibody Arrays Using Thermally Responsive Elastin Fusion Proteins, J. Am. Chem. Soc., 128, 676-677, 2006. pdf

116. Catherine Mee-Hie Cho, Ashok Mulchandani, and Wilfred Chen, Functional Analysis of organophosphorus hydrolase variants with high degradation activity toward organophosphate pesticides, Protein Engineering, Design, and Selection, 19, 99-105, 2006.  pdf

115. Priti Mulchandani, Wilfred Chen and Ashok Mulchandani, Microbial Biosensor for Direct Determination of Nitrophenyl Substituted Organophosphates Using Recombinant Moraxella sp., Analytica Chimica Acta, 568, 217-221, 2006. pdf

114. Yu Lei, Wilfred Chen and Ashok Mulchandani, Microbial Biosensors: A Review, Analytica Chimica Act, 568, 200-210, 2006. pdf

113. Cindy H. Wu, Thomas K. Wood, Ashok Mulchandani, and Wilfred Chen, Engineering plant-microbe symbiosis for rhizoremediation of heavy metal, Appl. Environ. Microbiol., 72, 1129-1134, 2006. pdf

112. Alberto Sanchez Arribas, Terannie Vazquez, Joseph Wang, Ashok Mulchandani, and Wilfred Chen, Electrochemical and optical bioassays of nerve agents based on the organophosphorus-hydrolase mediated growth of cupric ferrocyanide nanoparticles, Electrochemistry Communications, 7, 1371–1374, 2005. pdf

111. Kanchan A. Joshi, Marek Prouza, Maxwell Kum, Joseph Wang, Robert Haddon, Wilfred Chen, and Ashok Mulchandani, Direct detection of V-type nerve agents using a carbon nanotubes and OPH modified amperometric enzyme electrode, Anal. Chem., 78, 331-336, 2006. pdf

110. Zihui Meng, Wilfred Chen, and Ashok Mulchandani, Removal of estrogenic pollutants from contaminated waters using molecularly imprinted polymers, Environ. Sci. Technol., 39, 8958-8962, 2005. pdf

109. Aijun Wang, Andre M. Salazar, Marylynn V. Yates, Ashok Mulchandani, and Wilfred Chen, Visualization and detection of infectious coxsackievirus replication using a combined cell culture-molecular beacon assay, Appl. Environ. Microbiol., 71, 8397-8401, 2005. pdf

108. Yu Lei, Priti Mulchandani, Wilfred Chen and Ashok Mulchandani, Highly sensitive and selective amperometric microbial biosensor for direct determination of p-nitrophenyl substituted organophosphate nerve agents, Environ. Sci. Technol., 39, 8853-8857, 2005. pdf

107. Cory O’Neil, Derek Beving, Wilfred Chen, and Yushan Yan, Durability of hydrophilic and antimicrobial zeolite coatings for gravity-independent water separation, AIHCE J., 52, 1157-1161, 2006. pdf

106. Khaled H. Abd El Galil, M. A. El Sokkary, S. M. Kheira, Andre M. Salazar, Marylynn V. Yates, Wilfred Chen and Ashok Mulchandani, Real-Time Nucleic Acid Sequence-Based Amplification (NASBA) assay for the Detection of Hepatitis A virus, Appl. Environ. Microbiol., 71, 7113-7116, 2005. pdf

105. Lianbin Xu, Wilfred Chen, Ashok Mulchandani, Yushan Yan, Reversible Superhydrophobic to Superhydrophilic Conversion of Conducting Polymer Film, Angewandte Chemie International Edition, 44, 6009 –6012, 2005. pdf

104. Jan Kostal, Giridhar Prabhukumar, U Loi Lao, Alin Chen, Mark Matsumoto, Ashok Mulchandani, and Wilfred Chen,  Customizable Biopolymers for Heavy Metal Remediation, J. Nanoparticle Research, 7, 517-523, 2005. pdf (Invited contribution)

103. Gunjan Goel, Ashwani Kumar, A.K. Puniya, Wilfred Chen, and Kishan Singh, Molecular beacons: A multitask probe, J. Appl. Microbiol., 99, 435-442, 2005. pdf

102. Yu Lei, Ashok Mulchandani, and Wilfred Chen, Improved Degradation of Organophosphorus Nerve Agents and p-Nitrophenol by Pseudomonas putida JS444 with Surface-Expressed Organophosphorus Hydrolase, Biotechnol. Prog., 21, 678-681, 2005. pdf

101. Lingyun Rui, Li Cao, Wilfred Chen, Kenneth F. Reardon, and Thomas K. Wood, Protein Engineering of Epoxide Hydrolase from Agrobacterium radiobacter AD1 for Enhanced Activity and Enantioselective Production of (R)-1-Phenylethane-1,2-Diol, Appl. Environ. Microbiol., 71, 3995-4003, 2005. pdf

100. Aijun A. Wang, Wilfred Chen, and Ashok Mulchandani, Detoxification of organophosphate nerve agents by immobilized dual functional biocatalysts in a cellulose hollow fiber bioreactor, Biotechnol. Bioeng., 91, 379-386, 2005. pdf

99. Wilfred Chen, Ashok Mulchandani, and Marc Deshusses, Environmental Biotechnology: Challenges and Opportunities for Chemical Engineers, AICHE J., 51, 690-695, 2005. pdf

98. Wilfred Chen and Ashok Mulchandani. Engineering of Improved Biocatalysts in Bioremediation. Soil Biology, Volume 2, Biodegradation and Bioremeidation. A. Singh and O.P. Ward (Ed.). Springer-Verlag Berlin Heidelberg. Pp. 235-250, 2004.

97. Jae-Young Kim, Sean O’Malley, Ashok Mulchandani, and Wilfred Chen, Genetically engineered elastin-protein A fusion as a universal platform for homogeneous, phase-separation immunoassay, Anal. Chem., 77, 2318-2322, 2005. pdf

96. Jae-Young Kim, Ashok Mulchandani, and Wilfred Chen, Temperature-triggered purification of antibodies, Biotechnol. Bioeng., 90, 373-379, 2005. pdf

95. Kumaran Ramanathan, Mangesh A. Bangar, Minhee Yun, Wilfred Chen, Nosang V.  Myung, and Ashok Mulchandani, Bioaffinity Sensing Using Biologically-Functionalized Conducting Polymer Nanowire, J. Am. Chem. Soc., 127, 496-497, 2005. pdf

94. Priti Mulchandani, Carlos Hangtarer, Yu Lei, Wilfred Chen, and Ashok Mulchandani, Amperometric microbial biosensor for p-nitrophenol using Moraxella sp.-modified carbon paste electrode, Biosensors and Bioelectronics, 21, 523-527, 2005. pdf

93. Yu Lei, Priti Mulchandani, Wilfred Chen and Ashok Mulchandani, Direct determination of p-nitrophenyl substituent organophosphorus nerve agents using recombinant pseudomonas putida JS444-modified clark oxygen electrode, J. Agri. Food Chem., 53, 524-527, 2005. pdf

 92. Randhir Prakash Deo, Joseph Wang, Ines Block, Ashok Mulchandani, Kanchan A. Joshi, Marek Trojanowicz, Fritz Scholz, Wilfred Chen, and Yuehe Lin, Determination of organophosphate pesticides at a carbon nanotube/organophosphorus hydrolase electrochemical biosensor, Analytica Chimica Acta, 530, 185-189, 2005. pdf

 91. Lingyun Rui, Li Cao, Wilfred Chen, Kenneth F. Reardon, and Thomas K. Wood, Active Site Engineering of the Epoxide Hydrolase from Agrobacterium radiobacter AD1 to Enhance Aerobic Mineralization of cis-1,2-Dichloroethylene in Cells Expressing an Evolved Toluene ortho Monooxygenase, J. Biol. Chem., 279, 46810-46817, 2004. pdf

 90. Erica S. Forzani, Haiqian Q. Zhang, Wilfred Chen and Nongjian Tao, Detection of heavy metal ions in drinking water using a high-resolution differential Surface Plasmon Resonance Sensor, Environ. Sci. Technol., 39, 1257-1262, 2005. pdf

 89. Andrew M. P. McDonnell, Derek Beving, Albert Wang, Wilfred Chen, and Yushan Yan, Hydrophilic and antimicrobial zeolite coatings for gravity-independent water separation, Adv. Funct. Mat., 15, 336-340, 2005. pdf

 88. Kanchan A. Joshi, Joseph Wang, Robert Haddon, Wilfred Chen and Ashok Mulchandani., A disposable biosensor for organophosphorus nerve agents based on carbon nanotubes modified thick film strip electrode, Electroanalysis, 17, 54-58, 2005. pdf

87. Mark Shimazu, Wilfred Chen, Ashok Mulchandani. Biological detoxification of organophosphate pesticides.  In Pesticide Decontamination and Detoxification, Gan, J.J., Zhu, P.C., Aust, S.D. and Lemley, A.T. (eds.). American Chemical Society Symposium Series, Vol. 863, 25-36, 2004.

86. Mark Shimazu, Wilfred Chen, Ashok Mulchandani. Biodegradation of organophosphate nerve agents. Phosphorus in Environmental Technologies: Principles and Applications. Eugenia Valsami-Jones (Ed.). IWA Publishing, London, UK. pp. 629-642, 2004.

 85. Jianliang Zhang, Wensheng Lan, Chuanling Qiao, Hong Jiang, Ashok Mulchandani, and Wilfred Chen, Bioremediation of organophosphorus pesticides by surface-expressed carboxylesterase from mosquito on Escherichia coli, Biotechnol. Prog., 20, 1567-1571, 2004. pdf

 84. Kumaran Ramanathan, Mangesh Bangar, Minhee Yun, Wilfred Chen, Ashok Mulchandani, and Nosang V. Myung, Individually addressable conducting-polymer nanowires array, Nano Letters, 4, 1237-1239, 2004. pdf

 83. Jan Kostal, Rosanna Yang, Cindy H. Wu, Ashok Mulchandani, and Wilfred Chen, Enhanced Arsenic Accumulation in Engineered Bacterial Cells Expressing ArsR, Appl. Environ. Microbiol., 70, 4582-4587, 2004. pdf

 82. Kanchan A. Joshi, Prem C. Pandey, Wilfred Chen, and Ashok Mulchandani, Ormosil encapsulated pyrroloquinoline quinone-modified electrochemical sensor for thiols, Electroanalysis, 16, 1938-1943, 2004. pdf

 81. Khaled H. Abd El Galil, M. A. El Sokkary, S. M. Kheira, Andre M. Salazar, Marylynn V. Yates, Wilfred Chen and Ashok Mulchandani, Combined immunomagnetic separation-molecular beacon-reverse transcription-PCR assay for detection of Hepatitis A virus from environmental samples, Appl. Environ. Microbiol., 70, 4371-4374, 2004. pdf

80. Yu Lei, Priti Mulchandani, Wilfred Chen, Joseph Wang, Ashok Mulchandani, Arthrobacter sp. JS443-Based Whole Cell Amperometric Biosensor for p-Nitrophenol, Electroanalysis, 16, 2030-2034, 2004. pdf

79. Giridhar Prabhukumar, Mark Matsumoto, Ashok Mulchandani, and Wilfred Chen, Cadmium Removal from Contaminated Soil by Tunable Biopolymers, Environ. Sci. Tech., 38, 3148-3152, 2004. pdf

 78. Catherine Mee-Hie Cho, Ashok Mulchandani and Wilfred Chen, Altering the Substrate Specificity of Organophosphorus Hydrolase for Enhanced Hydrolysis of Chlorpyrifos, Appl. Environ. Microbiol., 70, 4681-4685, 2004. pdf

 77. Joseph Wang, Gang Chen, Alexander Muck Jr., Madhu Prakash Chatrathi, Ashok Mulchandani, Wilfred Chen, Microchip enzymatic assay of organophosphate nerve agents, Analytica Chimica Acta, 505, 183–187, 2004. pdf

 76. Yu Lei, Priti Mulchandani, Wilfred Chen, Joseph Wang and Ashok Mulchandani, Whole cell-enzyme hybrid amperometric biosensor for organophosphorous nerve agents, Biotechnol. Bioeng., 85, 706-713, 2004. pdf

75. M. Thust, A., Mulchandani, J. Wang, M. Arzdorf, P. Mulchandani, W. Chen, and M.J. Schoning, Field-effect enzyme sensor for the detection of pesticides, TECHNISCHES MESSEN, 70, 561-564, 2003.

 74. Jan Kostal, Ashok Mulchandani, and Wilfred Chen, Affinity purification of plasmid DNA by temperature-triggered precipitation, Biotechnol. Bioeng., 85, 293-297, 2004. pdf

 73. Jae-Young Kim, Ashok Mulchandani, and Wilfred Chen, An immunoassay for atrazine using tunable immunosorbent, Anal. Biochem., 322, 251-256, 2003. pdf

 72. Michael. J. Schoning, Michael Arzdorf, Priti Mulchandani, Wilfred Chen, and Ashok Mulchandani, Towards a capacitive enzyme sensor for direct determination of organophosphorus pesticides: Fundamental studies and aspects of development, Sensor, 3, 119-127, 2003. pdf

 71. Zhaohui Xu, Ashok Mulchandani, and Wilfred Chen, Detection of Benzene, Toluene, Ethyl Benzene, and Xylenes (BTEX) Using Toluene Dioxygenase-Peroxidase Coupling Reactions, Biotechnol. Prog., 19, 1812-1815, 2003. pdf

 70. Jan Kostal, Ashok Mulchandani, Katie E. Gropp, and Wilfred Chen, A temperature responsive biopolymer for mercury removal, Environ. Sci. Tech., 37, 4457-4462, 2003. pdf

 69. Sergi Morais,  Sean O’Malley, Wilfred Chen, and Ashok Mulchandani,  A tubulin-based fluorescence polarization bioassay for paclitaxel. Anal. Biochem., 321, 44-49, 2003. pdf

 68. Mark Shimazu, Anne Nguyen, Ashok Mulchandani, and Wilfred Chen, Cell surface display of OPH in Pseudomonas putida using an ice-nucleation protein anchor, Biotechnol. Prog., 19, 1612-1614, 2003. pdf

 67. Sergi Morais, Prem C. Pandey, Wilfred Chen and Ashok Mulchandani, A novel bioassay for screening and quantification of taxanes. Chem. Comm., 10, 1188-1189, 2003. pdf

66. Micheal J. Schoning, Micheal Arzdorf, Priti Mulchandani, Wilfred Chen, and Ashok Mulchandani, A capacitive field-effect sensor for the direct determination of organophosphorus pesticides, Sensors and Actuators B, 91, 92-97, 2003. pdf

65. Ibolya Bontidean, Josefin Ahlqvist, Ashok Mulchandani, Wilfred Chen, Weon Bae, Rajesh K. Mehar, Alessia Mortari, Elisabeth Csoregi, Novel synthetic phytochelatin-based capacitive biosensor for heavy metal ion detection, Biosensor & Bioelectronics, 18, 547-553, 2003. pdf

64. Weon Bae, Cindy Wu, Jan Kostal, Ashok Mulchandai, and Wilfred Chen, Enhanced mercury biosorption by bacterial cells with surface-displayed MerR, Appl. Environ. Microbiol., 69, 3176-3180, 2003. pdf

 63. Ryan Woodyer, Wilfred Chen, and Huimin Zhao, Outrunning Nature: Directed Evolution of Superior Biocatalysts, J. Chem. Edu., 81, 126-133, 2004. (Invited Contribution) pdf

62. Hana Stiborova, Jan Kostal, Ashok Mulchandani and Wilfred Chen, One-step metal affinity purification of histidine-tagged proteins by temperature-triggered precipitation, Biotechnol. Bioeng., 82, 605-611, 2003. pdf

61. Yu Lei, Priti Mulchandani, Wilfred Chen, Joseph Wang and Ashok Mulchandani, A Microbial Biosensor for p-Nitrophenol Using Arthrobacter Sp., Electroanalysis, 15, 1160-1164, 2003. pdf

60. Mark Shimazu, Ashok Mulchandani, and Wilfred Chen, Thermally Triggered Purification and Immobilization of Elastin-OPH Fusion, Biotechnol. Bioeng., 81, 74-79, 2003. pdf

59. Joseph Wang, Robin Krause, Kirstin Block, Mustafa Musameh, Ashok Mulchandani, Priti Mulchandani, Wilfred Chen, Michael J. Schöning, Dual amperometric-potentiometric biosensor detection system for monitoring organophosphorus neurotoxins, Analytica Chimica Acta, 469, 197-203, 2002. pdf

58. Wilfred Chen and George Georgiou, Cell-Surface Display of Heterologous Proteins: From High Throughput Screening to Environmental Applications, Biotechnol. Bioeng., 79, 469-503, 2002. (Invited Contribution) pdf

57. Priti Mulchandani, Yu Lei, Wilfred Chen, Joseph Wang and Ashok Mulchandani, A microbial biosensor for p-nitrophenol using Moraxella sp., Analytica Chimica Acta, 470, 79-86, 2002. pdf

56. Zhaohui Xu, Weon Bae, Ashok Mulchandani, Rajesh K. Mehra, and Wilfred Chen, Heavy metal removal by novel CBD-EC20 sorbents immobilized on cellulose, Biomacromolecules, 3, 462-465, 2002. pdf

55. Catherine M-H. Cho, Ashok Mulchandani, and Wilfred Chen, Bacterial cell surface display of organophosphorus hydrolase for selective screening of improved hydrolysis of organophosphate nerve agents, Appl. Environ. Microbiol., 68, 2026-2030, 2002. pdf

54. Aijun A. Wang, Ashok Mulchandani, and Wilfred Chen, Specific adhesion to cellulose and hydrolysis of organophosphate nerve agents by a genetically engineered E. coli with surface-expressed cellulose-binding domain and organophosphorus hydrolase, Appl. Environ. Microbiol., 68, 1684-1689, 2002. pdf

53. Weon Bae, Rajesh Mehra, Ashok Mulchandani, and Wilfred Chen, Genetic engineering of Escherichia coli for enhanced bioaccumulation of mercury, Appl. Environ. Microbiol., 67, 5335-5338, 2001. pdf

52. Sung Hyo Chough, Ashok Mulchandani, Priti Mulchandani, Wilfred Chen, Joseph Wang and Kim R. Rogers, Organophosphorus hydrolase-based electrode for determination of organophosphate pesticides, Electroanalysis, 14, 273-276, 2002. pdf  

51. Weon Bae, Ashok Mulchandani, and Wilfred Chen, Cell surface display of synthetic phytochelatins using ice nucleation protein for enhanced heavy-metal bioaccumulation, J. Inorg. Biochem., 88, 223-227, 2002. (Invited Contribution) pdf

50. Mark Shimazu, Ashok Mulchandani, and Wilfred Chen, Simultaneous Degradation of Organophosphorus Pesticides and p-nitrophenol by a Genetically Engineered Moraxella sp. with Surface-Expressed Organophosphorus Hydrolase, Biotechnol. Bioeng., 76, 318-324, 2001. pdf

49. Priti Mulchandani, Wilfred Chen, Ashok Mulchandani, Flow Injection Amperometric Enzyme Biosensor for Direct Determination of Organophosphate Nerve Agents, Environ. Sci. Tech., 35, 2562-2565, 2001. pdf

48. Joseph Wang, Madhu P. Chatrathi, Ashok Mulchandani, and Wilfred Chen, Capillary Electrophoresis Microchips for Rapid Separation and Detection of Organophosphate Nerve Agents, Anal. Chem., 73, 1804-1808, 2001. pdf

47. Ashok Mulchandani, Wilfred Chen, Priti Mulchandani, Joseph Wang, and Kim R. Rogers, Biosesnors for Direct Determination of Organophosphate Pesticides, Biosens. Bioelectron., 16, 225-230, 2001. (Invited Contribution) pdf

46. Priti Mulchandani, Wilfred Chen, Ashok Mulchandani, Joseph Wang and Liang Chen, Amperometric Microbial Biosensor for Direct Determination of Organophosphate Nerve Agents using Recombinant Moraxella sp. with Surface Expressed Organophosphorus Hydrolase, Biosens. Bioelectron., 16, 433-437, 2001. (Invited Contribution)  pdf

45. Jan Kostal, Ashok Mulchandani, and Wilfred Chen, Tunable Biopolymers for Heavy Metal Removal, Macromolecules, 34, 2257-2261, 2001. pdf

44. Aijun Wang, Ashok Mulchandani, and Wilfred Chen, Whole Cell Immobilization using Surface-expressed Cellulose-Binding Domain, Biotechnol. Prog., 17, 407-411, 2001. pdf

43. Richard Richins and Wilfred Chen, Effects of FIS Overexpression on Cell Growth, rRNA Synthesis and Ribosome Content in Escherichia coli, Biotechnol. Prog., 17, 252-257, 2001. pdf

42. Ashok Mulchandani, Priti Mulchandani, Wilfred Chen, Joseph Wang, Liang Chen. Field-deployable enzyme electrodes for direct monitoring of organophosphate nerve agents. In: Enzymes in Heteroatom Chemistry: Green solutions for chemical problems. (Zwanenburg, B. et al., eds.), Kluwer Academic Publishers. The Netherlands, pp.287-296, 2000.

41. Wilfred Chen, Richard D. Richins, Priti Mulchandani, Irina Kaneva, and Ashok Mulchandani. Biodegradation of Organophosphorus Nerve Agents By Surface Expressed Organophosphorus Hydrolase. In: Enzymes in heteroatom chemistry: Green solutions for chemical reactions. (Zwanenburg, B. et al., eds.), Kluwer Academic Publishers. The Netherlands, pp. 211-222, 2000.

40. Ayman Mansee, Wilfred Chen, and Ashok Mulchandani, Biodetoxification of coumaphos insecticide using immobilized Escherichia coli expressing organophosphorus hydrolase enzyme on cell surface, Biotechnol. Bioprocess Eng., 5, 436-440, 2000. pdf

39. Mark Shimazu, Ashok Mulchandani, and Wilfred Chen, Cell Surface Display of Organophosphorus Hydrolase Using Ice Nucleation Protein, Biotechnol. Prog., 17, 76-80, 2001. pdf

38. Nathalie Fortin, Ashok Mulchandani, and Wilfred Chen, Use of real-time polymerase chain reaction and molecular beacon for the detection of Escherichia coli O157:H7, Anal. Biochem., 289, 281-288, 2001. pdf

37. Wilfred Chen, Ashok Mulchandani, and Joseph DeFrank, Biodegradation of Chemical Weapons, in Encyclopedia of Environmental Microbiology, John Wiley & Sons (invited contribution), 844-852, 2001.

36. Weon Bae, Wilfred Chen, Ashok Mulchandani, and Rajesh Mehra, Enhanced Bioaccumulation of Heavy Metal by Bacterial Cells Displaying Synthetic Phytochelatins, Biotechnol. Bioeng., 70, 518-524, 2000. pdf

35. Wilfred Chen, Grisselle Martinez and Ashok Mulchandani, Molecular Beacons: A New Approach for Detecting Salmonella Species, Sadik, O.A. and Mulchandani, A. (eds.), ACS Symposium Series No. 762, ACS, Washington, DC, pp.292-298, 2000.

34. Richard D. Richins, Ashok Mulchandani, and Wilfred Chen, Expression, Immobilization, and Enzymatic Characterization of Cellulose-Binding Domain-Organophosphorus Hydrolase Fusion Proteins, Biotechnol. Bioeng., 69, 591-596, 2000. pdf

33. Wilfred Chen, Grisselle Martinez, and Ashok Mulchandani, Molecular Beacons: A Real-Time Polymerase Chain Reaction Assay for Detecting Salmonella, Anal. Biochem., 280, 166-172, 2000. pdf

 32. Wilfred Chen, Fredi Bruhlman, Kelvin H. Lee and Marc A. Deshusses, Whole Cell Catalysis, in Encyclopedia of Catalysis, John Wiley & Sons (invited contribution), Vol. 6, 658-680, 2003.

31. Fredi Bruhlmann and Wilfred Chen, Transformation of Polychlorinated Biphenyl by a Novel BphA variant through the meta-cleavage pathway, FEMS Microbiol. Lett., 179, 203-208, 1999. pdf   

30. Joseph Wang, Liang Chen, Ashok Mulchandani, Priti Mulchandani, and Wilfred Chen, Remote Biosensor for in-situ Monitoring of Organophosphate Nerve Agents. Electroanalysis 11, 866-869, 1999. pdf

29. Wilfred Chen and Ashok Mulchandani, Detoxification of pesticides, Biofutur, 187, 41-43, 1999.

28. Ashok Mulchandani, Priti Mulchandani, Wilfred Chen, Joseph Wang, and Liang Chen, Amperometric Thick-Film Strip Electrodes for Monitoring Organophosphate Nerve Agents Based on Immobilized Organophosphorus Hydrolase, Anal. Chem., 71, 2246-2249, 1999. pdf

27. Kim R. Rogers, Y. Wang, A. Mulchandani, P. Mulchandani, and Wilfred Chen, Organophosphorus Hydrolase-Based Assay for Organophosphate Pesticides, Biotechnol. Prog., 15, 517-521, 1999. pdf

26. Wilfred Chen, Fredi Bruhlmann, Richard Richins, and Ashok Mulchandani, Engineering of Improved Enzymes and Microbes for Bioremediation, Curr. Opin. Biotechnol., 10, 137-141, 1999. pdf

25. Fredi Bruhlmann and Wilfred Chen, Tuning Biphenyl Dioxygenases for Extended Substrate Specificity, Biotechnol. Bioeng., 63, 544-551, 1999. pdf

24. Ashok Mulchandani, Shengtian Pan and Wilfred Chen, Fiber-Optic Enzyme Biosensor for Direct Determination of Organophosphate Nerve Agents, Biotechnol. Prog., 15, 130-134, 1999. pdf

23. Leila H. Choe, Wilfred Chen and Kelvin H. Lee, Proteome Analysis of Fis Overproduction in Escherichia coli, Electrophoresis, 20, 798-805, 1999. pdf

22. Ashok Mulchandani, Irina Kaneva and Wilfred Chen, Detoxification of Organophosphate Nerve Agents by Immobilized Escherichia coli with Surface-Expressed Organophosphorus Hydrolase, Biotechnol. Bioeng., 63, 216-223, 1999. pdf

21. Ashok Mulchandani, Priti Mulchandani and Wilfred Chen, Enzyme Biosensor for Determination of Organophosphates, Field Analytical Chemistry and Technology, 2, 363-369, 1998. pdf

20. Irina Kaneva, Priti Mulchandani, Richard Richins, Ashok Mulchandani, and Wilfred Chen. 1998. Biodetoxification and Monitoring of Organophosphorus Nerve Agents by surface-Expressed Organophosphorus Hydrolase. In Proceedings of the 1997 ERDEC Scientific Conference on Chemical & Biological Defense Research, 18-21 November 1997, ERDEC-SP-063, 251-255.

19. Priti Mulchandani, Ashok Mulchandani, Irina Kaneva and Wilfred Chen, Biosensor for Direct Determination of Organophosphate Nerve Agents. 1. Potentiometric Enzyme Electrode, Biosens. Bioelectron., 14, 77-85, 1999. pdf

18. Ashok Mulchandani, Irina Kaneva and Wilfred Chen, Biosensor for Direct Determination of Organophsophate Nerve Agents Using Recombinant Escherichia coli with Surface-Expressed Organophosphorus Hydrolase. 2. Fiber-Optic Microbial Biosensor, Anal. Chem. 70, 5042-5046, 1998. pdf

17. Ashok Mulchandani, Priti Mulchandani, Irina Kaneva, and Wilfred Chen, Biosensor for Direct Determination of Organophosphate Nerve Agents Using Recombinant Escherichia coli with Surface-Expressed Organophosphorus Hydrolase. 1. Potentiometric Electrode, Anal. Chem., 70, 4140-4145, 1998. pdf

16. Ashok Mulchandani, Samir Chauhan, Irina Kaneva, Priti Mulchandani, and Wilfred Chen, A Potentiometric Microbial Biosensor for Direct Determination of Organophosphate Nerve Agents, Electroanalysis, 10, 733-737, 1998. pdf

15. Irina Kaneva, Ashok Mulchandani, and Wilfred Chen, Factors Influencing Parathion Degradation by Recombinant E. coli with Surface-Expressed Organophosphorus Hydrolase, Biotechnol. Prog., 14, 275-278, 1998. pdf

14. Wilfred Chen and Ashok Mulchandani, The Use of Live Biocatalysts for Pesticide Detoxification, Trends in Biotechnology, 16, 71-76, 1998. pdf

13. Richard Richins, Irina Kaneva, Ashok Mulchandani, and Wilfred Chen, Biodegradation of Organophosphorus Pesticides by Surface-Expressed Organophosphorus Hydrolase, Nature Biotechnology, 15, 984-987, 1997. pdf

12. Richard Richins, Tin Htay, Pauli Kallio, and Wilfred Chen, Elevated Fis Expression Enhances Recombinant Protein Synthesis in Escherichia coli, Biotechnol. Bioeng., 56, 138-144, 1997. pdf

11. Neilay Dedhia, Wilfred Chen and James E. Bailey, Design of Expression Systems for Metabolic Engineering: Coordinated Synthesis and Degradation of Glycogen, Biotechnol. Bioeng., 55, 419-426, 1997. pdf

10. Marc A. Deshusses, Wilfred Chen, Ashok Mulchandani, and Irving J. Dunn., Innovative Bioreactors, Curr. Opin. Biotechnol., 8, 165-168, 1997. pdf

9. Neilay N.Dedhia, Richard Richins, Archie Mesina, and Wilfred Chen, Improvement in Recombinant Protein Production in ppGpp-Deficient Escherichia coli, Biotechnol. Bioeng., 53, 379-386, 1997. pdf

8. Wilfred Chen, Pauli Kallio and James Bailey, Process Characterization of a Novel Cross-Regulation System for Cloned Protein Production in Escherichia coli, Biotechnol. Prog., 11, 397-402, 1995. pdf

7. Wilfred Chen, Pauli Kallio, and James E. Bailey, Cross-Regulation of Gene Expression in Recombinant Cells , US Patent Number 5,416,800, 1995.

6. Wilfred Chen, Dallas E. Hughes and James E. Bailey, Intracellular Expression of Vitreoscilla Hemoglobin Alters Aerobic Metabolism of Saccharomyces cerevisiae, Biotechnol. Prog., 10, 308-313, 1994. pdf

5. Wilfred Chen and James E. Bailey, Application of the Cross-Regulation System as a Metabolic Switch, Biotechnol. Bioeng., 43, 1190-1193, 1994. pdf

4. Wilfred Chen, Pauli Kallio, S.B. Lee,. and James E. Bailey, Invention of a Useful Transcriptional Regulation System Using Mechanistic Mathematic Modelling, p. 155-158, in Modern Trends in Biothermokinetics (S. Schuster, M. Rigoulet, R. Ouhabi, and J.P. Mazat, Eds), Plenum Press, New York, U.S.A., 1993.

3. Wilfred Chen, Pauli Kallio and James E. Bailey, Construction and Characterization of a Novel Cross-Regulation System for Regulating Cloned-Gene Expression in E. coli, Gene, 130, 15-22, 1993. pdf

2. Neilay Dedhia, Thomas Hottiger, Wilfred Chen, and James E. Bailey, Genetic Manipulation of Central Carbon Metabolism in Escherichia coli, p. 59-62 in Harnessing Biotechnology for the 21st Century (M. R. Ladisch and A. Bose, Eds), American Chemical Society, New York, 1992.

1. Wilfred Chen, James E. Bailey and Sun Bok Lee, Molecular Design of Expression Systems: Comparison of Different Repressor Control Configurations Using Molecular Mechanism Models, Biotechnol. Bioeng., 38, 679-687, 1991. pdf


 
 

Department of Chemical  and Biomolecular Engineering, Colburn Lab 224, University of Delaware, Newark, DE 19716

Maintained by Wilfred Chen